De-Plasma™ II

De-Plasma™ II is a safe, potent reagent which can be used to prevent or eliminate Mycoplasma contamination. It consists of a proprietary mixture of five antimicrobial compounds with three separate anti-Mycoplasma targets. It is non-toxic to most cell lines with an ED50 ten times larger than the treatment concentration.
Mycoplasma belong to a unique genus of bacteria that lack cell walls. Their small size and ubiquitous distribution account for their ability to go undetected in seemingly sterile cell cultures, but ultimately cause alterations in cell physiology and metabolism, leading to inaccurate experimental conclusions.
De-Plasma™ II is an antibiotic cocktail comprised of one macrolide, one quinolone, and one tetracycline compound, each of these groups have shown they are highly active against Mycoplasmas. This mixture offers higher potency and is effective against all known Mycoplasma species and related non cell-walled prokaryotes.

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Background

The mechanism of action for each of the antibiotics in De-Plasma II are different. Tetracyclines and macrolides block protein synthesis by interfering with translation at the prokaryotic ribosome. Quinolones inhibit bacterial DNA gyrase and topoisomerase IV, blocking DNA replication. Eukaryotic cells do not contain DNA gyrase or topoisomerase IV and are therefore unaffected by De-Plasma II.