DBCO-dPEG®₄-MAL

DBCO-dPEG®4-MAL, product number 10592, is a short, versatile discrete polyethylene glycol (dPEG®) crosslinking reagent. The dibenzylcyclooctyne (DBCO) moiety permits strain promoted azide-alkyne cycloaddition (SPAAC), also known as "copper free click chemistry." The maleimidopropyl (MAL) functional group forms a thiol-ether linkage with sulfhydryl groups. The short, single molecular weight dPEG® spacer is hydrophilic and non-immunogenic.

The copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) discovered by K. Barry Sharpless and coworkers is one of the best known click chemistry reactions. However, copper toxicity makes this reaction problematic for in vivo labeling experiments. Carolyn Bertozzi and coworkers developed SPAAC as a way to avoid copper's toxic effects on living cells using highly strained cyclooctyne. Developments since the initial publication show that reagents based on DBCO provide excellent, highly selective reactivity with azides, forming a triazole product.

Maleimide groups are widely popular for their ability to react rapidly with sulfhydryl groups via a Michael addition to form thioether bonds.

OurdPEG® products clearly differ from traditional, polymeric polyethylene glycol (PEG). Traditional PEG is a disperse polymer product with a Poisson distribution of chain lengths and molecular weights. The intractable mixture of chain lengths and molecular weights in traditional PEG products creates significant analytical challenges. In contrast, our dPEG® products are single molecular weight compounds. Each dPEG® product contains a single, discrete chain length and is a single, molecular weight.

DBCO-dPEG®4-MAL provides the benefits of PEG (increased water solubility, non-immunogenicity) without the analytical headaches of PEG dispersity. PN10592 is one of three products with these functional groups, each of which differs by dPEG® chain length. The other two products in this series are PN10593, DBCO-dPEG®12-MAL, and PN10594, DBCO-dPEG®24-MAL. If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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