DBCO-dPEG®₄-TFP ester

DBCO-dPEG®4-TFP ester, product number 11362, is a heterobifunctional, bioorthogonal crosslinker. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds. The dibenzylcyclooctyne (DBCO) moiety reacts selectively with azides via strain-promoted azide-alkyne cycloaddition (SPAAC) to form triazole linkages. An amphiphilic, flexible, single molecular weight, discrete polyethylene glycol (dPEG®) spacer separates the two functional groups. The dPEG® spacer improves the water solubility of the conjugates.

Why use Copper-Free Click Chemistry?
The published discovery in 2004 of SPAAC, also known as "copper-free click chemistry," by Carolyn Bertozzi and coworkers, improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. Because copper is not part of the reaction, SPAAC reagents can label living cells. Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC.

What Makes dPEG® Products Different?
OurdPEG® reagents are superior to traditional, polymeric polyethylene glycol (PEG) reagents. Traditional PEG reagents are dispersed polymers (Đ > 1). Disperse PEG contains a complex mixture of different chain lengths and molecular weights in a Poisson distribution. In contrast, each dPEG® reagent from Quanta BioDesign, Ltd contains a single PEG compound. DBCO-dPEG®4-TFP ester simplifies the analysis of conjugates formed in the crosslinking reactions because the dPEG® crosslinker is a discrete chain length product with a single molecular weight.

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