Dabcyl Support 500A

Dabcyl Amidites and Supports

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

Dabcyl phosphoramidites and solid supports:

A molecular beacon probe has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. Dabcyl CPGs and Dabcyl Amidites are used to prepare probes with the quencher blocking the 3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.