D-Luciferin K salt

- Overview
  - D-Luciferin is a firefly luciferase substrate. Its quantum efficiency is 0.88, which is 20 times that of Luminol. The reaction mechanism is shown below. First, Luciferin reacts with ATP by luciferase in the presence of magnesium ion, then it is oxidized to form dioxetane structure and emits yellow-green light. Luciferin-luciferase luminescence is used for ATP monitoring to determine cell viability and bacteria counting. It is also used for reporter gene assays.
    
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- Properties
  - Categories
    
    Ion Analysis
    
    Alternative Name
    
    (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, potassium salt
    
    CAS Number
    
    115144-35-9
    
    Molecular Formula
    
    C11H7N2O3S2K
    
    Molecular Weight
    
    318.42
    
    Appearance
    
    Pale yellow or yellow powder
    
    Purity
    
    ≥98.0% (HPLC)
    
    Storage
    
    -20°C, Protect from light and moisture, under nitrogen gas
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application
    
    Specialty Chemicals
- Reference
  - 1) V. T. Nguyen, et al., Firefly Luciferase Luminescence Assays Using Scintillation Counters for Quantitation in Transfected Mammalian Cells. Anal Biochem. 1988;171:404-408.
    
    2) S. P. Crouch, et al., The Use of ATP Bioluminescence as a Measure of Cell Proliferation and Cytotoxicity. J Immunol Methods. 1993;160:81-88.
    
    3) S. R. Ford, et al., Improvements in the Application of Firefly Luciferase Assays. Methods Mol Biol. 1998;102:3-20.

MSDS

D-Luciferin K salt

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