Cy3 Bis NHS ester

- Overview
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    Background
    
    Cy3 Bis NHS ester is a reactive fluorescent dye for general labeling of protein amine groups. Cy3 Bis NHS ester includes two reactive groups on one dye molecule. The N-hydroxysuccinimde (NHS) ester is the most popular amine reactive group, used for capturing the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Appling NHS chemistry allows one to conjugate a variety of fluorescent probes (e.g. Cy3, a traditional orange-fluorescent label which is highly water soluble) to primary amines. Thus, target biological compounds can be visualized by Cy3 fluorescence (~550 nm excitation, ~570 nm emission) monitored by various fluorometers, imagers, and microscopes.
    
    1. Nanda JS, Lorsch JR. Labeling a protein with fluorophores using NHS ester derivitization. Methods in Enzymology, 2014, 536: 87-94.
    2. Dyla M, Andersen JL, Kjaergaard M, et al. Engineering a Prototypic P-type ATPase Listeria monocytogenes Ca2+-ATPase 1 for Single-Molecule FRET Studies. Bioconjugate Chemistry, 2016, 27(9): 2176-2187.
- Properties
  - Categories
    
    A reactive fluorescent dye for general labeling of protein amine groups
    
    Alternative Name
    
    sodium 1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-((1E,3E)-3-(1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
    
    Molecular Formula
    
    C43H49N4NaO14S2
    
    Molecular Weight
    
    932.99
    
    Purity
    
    98%
    
    Solubility
    
    insoluble in EtOH; ≥41 mg/mL in DMSO; ≥54.6 mg/mL in H2O
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CCCCCC(ON3C(CCC3=O)=O)=O)(C)C2=C/C=C/C4=[N+](CCCCCC(ON5C(CCC5=O)=O)=O)C(C=CC(S(=O)([O-])=[O-])=C6)=C6C4(C)C.[Na+]
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Cy3 Bis NHS ester

Background

Documents

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