Comet Assay Kit

The in vivo alkaline comet (single cell gel electrophoresis) assay is used for the detection of DNA strand breaks in cells or nuclei isolated from multiple tissues of animals, usually rodents, that have been exposed to potentially genotoxic material(s). The purpose of the comet assay is to identify substances that cause DNA damage. Under alkaline conditions (>pH 13), the comet assay can detect single and double stranded breaks, resulting transient DNA strand breaks that may be lethal to the cell, or may be fixed into a mutation resulting in a permanent viable change. The extent of DNA damage can be measured by electrophoresis after denaturing and unwinding procedures. The migration distance reflects the amount and size of DNA fragments. The DNA content in the tail (% tail DNA or % tail intensity) has been recommended to assess DNA damage.

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