Name: COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE)
Price: 485.00 USD

- Overview
  - 1ml of FITC-conjugated anti Myeloperoxidase-C2 (clone 8E6) and PE-conjugated anti CD22 (cloneRFB4) in PBS pH 7.2, 1% BSA, and 0.05% NaN3, approximately 50 tests.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Myeloperoxidase (MPO) is a glycoprotein present in the azurophil (primary) granules of myeloid cells, which appears in the myeloblast stage of myeloid cell differentiation. MPO is he most common functional protein of myeloid cells and is involved in the inflammatory response. It helps to kill microbes by breaking down peroxide in the presence of halide ions, contributing to the bactericidal function of granulocytes. The primary translation product of MPO undergoes glycosylation with production of the 89 kDa heme-free apopro-MPO form followed by incorporation of heme and conversion into the enzymatically active pro-MPO form. Subsequently, pro-MPO becomes targeted to azurophil granules where final processing occurs to produce mature dimeric MPO consisting of the 59-64 kDa MPO ?-chain and the 14 kDa MPO ?-chain.
    
    Precursor B-cells are surface-CD22 negative, but cytoplasmic CD22 positive. Mature B-lymphocytes.express CD22 also on their surface. The combined staining for MPO and CD22 allows the distinction of mature/immature myeloid cells and B-lymphocytes.
    
    The MPO-C2/CD22 COMBI-IC mAb permits the identification and enumeration of normal and malignant myelomonocytic cells and B lymphoid commited cells in human blood and bone marrow using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Clone
    
    8E6 and RFB4
    
    Conjugate
    
    FITC; PE
    
    Formulation
    
    PBS pH 7.2, 1 mg/ml BSA, 0.1% NaN3
    
    Storage
    
    Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8°C (DO NOT FREEZE!) and protected from prolonged exposure to light. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
    
    Note
    
    This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
    
    Regulatory Status
    
    CE Marking
    
    * For professional users only.
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    Permeabilization and Staining Procedure
    - In combination with our Permeabilization Kit FIX&PERM? (Cat. No. GAS-002) intracellular MPO-C2 and CD22 can be easily stained in cell suspensions.
    - For each sample to be analyzed add 50 µl of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube
    - Add 100 µl of Reagent A (Fixation Medium, stored and used at room temperature)
    - Incubate for 15 minutes at room temperature
    - Add 5ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g
    - Remove supernatant and add to cell pellet 100 µl Reagent B (Permeabilization Medium) and 20 µl of the MPO-C2/CD22 COMBI-IC monoclonal antibody conjugate
    - Vortex at low speed for 1-2 seconds
    - Incubate for 15 minutes at room temperature
    - Wash cells with phosphate buffered saline as described above
    - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2-8°C in the dark. Analyze
    fixed cells within 24 hours.
- Reference
  - 1. Andersson, E., Hellman, L., Gullberg, U. & Olsson, I. (1998) J Biol Chem 273, 4747-53.
    2. Braylan, R. C., Orfao, A., Borowitz, M. J. & Davis, B. H. (2001) Cytometry 46, 23-7
    
    3. Catovsky, D., Matutes, E., Buccheri, V., Shetty, V., Hanslip, J., Yoshida, N. & Morilla, R. (1991) Ann Hematol 62, 16-21.
    4. Cowland, J. B. & Borregaard, N. (1999) J Leukoc Biol 66, 989-95
    
    5. Groeneveld, K., te Marvelde, J. G., van den Beemd, M. W., Hooijkaas, H. & van Dongen, J. J. (1996) Leukemia 10, 1383-9
    6. Gullberg, U., Andersson, E., Garwicz, D., Lindmark, A. & Olsson, I. (1997) Eur J Haematol 58, 137-53.
    7. He, J. & Furmanski, P. (1995) Nature 373, 721-4.
    8. Imamura, N. (1998) Am J Hematol 58, 241-3.
    
    9. Knapp, W., Majdic, O. & Strobl, H. (1993) Recent Results Cancer Res 131, 31-40.
    
    10. Koeffler, H. P., Ranyard, J. & Pertcheck, M. (1985) Blood 65, 484-91.
    
    11. Konikova, E., Glasova, M., Kusenda, J. & Babusikova, O. (1998) Neoplasma 45, 282-91.
    12. Lanza, F., Latorraca, A., Moretti, S., Castagnari, B., Ferrari, L. & Castoldi, G. (1997) Cytometry 30, 134-44.
    13. Murao, S., Stevens, F. J., Ito, A. & Huberman, E. (1988) Proc Natl Acad Sci U S A 85, 1232-6.
    14. Nakase, K., Sartor, M. & Bradstock (1998) Cytometry 34, 198-202.
    15. Nauseef, W. M. (1990) Hematol Pathol 4, 165-78.

Datasheet

COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE)

COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE)

Background

We Also Recommend

anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified

anti-AAV VP1/VP2 mouse monoclonal, A69, lyophilized, purified

Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDyeTM 488 Conjugate