Chemically Defined Serum Replacement for High Density Culture

CDM-HD is a chemically defined, protein free, animal component free cGMP compliant serum replacement optimized for use in hollow fiber bioreactors.

The culture of cells at in-vivo like cell densities allows cells to generate their own micro-environment and reduces dependency on exogenous factors found in serum or serum-free mediums. However, commercial serum free mediums can be expensive, contain surfactants, and are each optimal for only a limited number of cell types.

The concept of CDM-HD is simple: produce a universal serum substitute that can take advantage of the simplified serum requirements for cells grown at high densities in hollow fiber bioreactor systems. CDM-HD represents a true breakthrough in cell culture ideology and technology. It represents the first time that a cell culture medium can be simplified and optimized for the unique hollow fiber bioreactor bio-environment.

Features
· Chemically defined
· Protein free
· Animal component free
· Dry powder
· Store at 4 degrees

Benefits
· Lot-to-lot consistency
· Purification of secreted products simplified
· Regulatory compliance simplified, no agents of unknown origin, mycoplasma, viruses
· Long shelf life
· Simple storage conditions

WHAT IS IN CDM-HD?
· Customized ratios of amino acids and metabolites
· Vitamins and co-factors
· Trace elements
· Micro-nutrients
· Extra buffering capacity
· Recombinant growth factors
· Carbohydrates, including 800 mg of glucose per liter final medium
· All low molecular weight components, nothing larger than 3 kD

WHAT IS NOT IN CDM-HD?
· Protein
· Hydrolysates
· Undefined reagents
· Animal-derived components
· Steroid hormones or peptide mitogens
· Phenol red
· Plurionic Detergent
· High molecular weight materials including synthetic polymers

Optimized for Hollow Fiber Bioreactors
· Increased buffering capacity maintains optimum conditions for protein and antibody production.
· No detergents or other cell membrane stabilizing agents that can interfere with assays or precipitations.
· Composition balanced for high-density culture.

Please contact us at for specific academic pricing.