-Cellstain-CytoRed Solution

-Cellstain-CytoRed Solution

Catalog Number:
CFA1108160DOJ
Mfr. No.:
C410-10
Price:
$369
  • Size:
    1 ml
    Quantity:
    Add to Cart:
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      • Overview

        • CytoRed is cell membrane permeable and accumulates inside of viable cells as resorufin. CytoRed has a much wider spectrum than BCECF or Calcein, so filters for fluorescein and rhodamine can also be used. The excitation and emission wavelengths of resorufin are 560 nm and 590 nm, respectively.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Cell Staining
          Molecular Formula
          C17H15NO5
          Molecular Weight
          313.31
          Appearance
          yellowish-orange solution
          Storage
          -20°C, protect from light
          Shipping
          with blue ice

          * For research use only

      • Applications
        • Application Description
          Staining Procedure
          1.Prepare 1 mM CytoRed solution with DMSO. Dilute it to prepare 10 μM CytoRed solution with culture medium or an appropriate buffer.a)
          2.Prepare a 1×105-1×106 cells/ml cell suspension and culture the cells in a chamber slide.
          3.Remove culture medium and wash cells with culture medium (PBS-Hanks medium, etc).
          4.Add CytoRed solution to the cells, and incubate the chamber at 37°C for 30 min to 1 hour.
          5.Remove the culture medium from cells and add new medium.b)
          6.Wash cells twice with PBS or an appropriate buffer.
          7.Observe the cells under a fluorescence microscope with 560 nm excitation and 590 nm emission filters.
          a) Incubate the MitoRed buffer solution at 37°C prior to adding to cells.
          b) For fixing after washing cells, add 10% formarin buffer and incubate for 15-20 min, and then wash with PBS.
      • Reference

        • 1) M. Ishiyama, et al., A Resorufin Derivative as a Fluorogenic Indicator for Cell Viability. Anal Sci. 1999;15:1025-1028.

    MSDS

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