Cell DNA Isolation Kit

- Overview
  - The DNA Isolation Kit (Cultured Cell) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial and viral DNA from cultured cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Isolate high molecular weight DNA for archiving or sensitive downstream applications
    High yield, High quality DNA (A260/A280 = 1.8-2.0)
    Sample: 0.5-1.5 x 10⁹ cultured cells
    Easily scalable to satisfy larger cultured cell sample volumes
    Minimal hands on time
    Convenient, simple and gentle reagent DNA precipitation method
    
    Storage
    
    dry at room temperature (15-25°C) for up to 2 years, RNase A should be stored at -20°C for extended periods
    
    * For Research Use Only
- Applications
  - Application
    
    Reagent DNA Extraction
    
    Application Description
    
    DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

Documents

PROTOCOL

Cell DNA Isolation Kit

Documents

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