Cell Counting Kit-F

Cell Counting Kit-F(CCK-F) is utilized for the fluorometric determination of living cell numbers. The amount of the fluorescent dye, calcein, hydrolyzed by esterases in cells is directly proportional to the number of viable cells in culture media. The 96-well microplate CCK-F assay has a detection range of less than 50 cells to more than 25,000 cells per well. Since esterases and phenol red in the culture medium interfere with the fluorescence measurement, replacing the cell culture medium with PBS is necessary prior to adding the Calcein-AM assay solution. The excitation and the emission wavelengths of calcein are 485 nm and 535 nm, respectively. An incubation of 10 to 30 minutes gives sufficient fluorecence intensity for the cell viability determination.

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