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Overview
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5(6)-CDCFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-CDCFDA yields the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this dye can be used as an acidic pH sensor.
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