Cartridge

Puri-Pak Cartridges & Barrels

Puri-Pak Cartridges & Barrels:

The Puri-PakTM has been developed by to purify oligonucleotides by DMT-ON method and to desalt oligonucleotides which have been purified by ion-exchange HPLC or Gel Electrophoresis. They are very effective in producing high purity oligonucleotides after synthesis.

The Puri-Pak cartridgesTM are suitable for the following applications:

Low scale and also 1.0 μmol scale purification of synthetic oligonucleotides, modified synthetic oligonucleotides carrying amino-terminals, biotin, phosphorothioate oligonucleotides, and 2’-OMe oligonucleotides.
Desalting of oligonucleotides.

Technical Details:

The Puri-Pak columns are designed for efficient and fast purification of DMT-ON oligonucleotides as well as for desalting of oligonucle­otides. They are recommended for single use where 25 OD units can be loaded per cartridge. The Puri-Pak columns can also efficiently purify crude oligonucleotides. Also, 5’-DMT-ON oligonucleotides can be separated cleanly from the shorter “fail” sequences. After NH3 deprotection of the oligonucleotide, the NH3 solution containing the dissolved oligo can be loaded directly onto the Puri-Pak column. We recommend washing the crude oligo with Ether prior to removal of the cleaved base protecting groups. The oligo should then be dissolved in either water or 0.1M triethylammonium acetate (1-3ml) and loaded onto the Puri-Pak column.