Cancer Signaling Phospho Antibody Array

The newly updated Cancer Signaling Antibody Array features 269 highly specific antibodies important to cancer signaling pathways. The array is designed for protein phosphorylation profiling in cancer cell and tissue samples from human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

The Cancer Signaling Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling, designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies for studying serine, threonine, and tyrosine phosphorylation specific sites. The 269 antibodies on this array allow researchers to examine key proteins from multiple cancer cellular pathways including AKT signaling, apoptosis, EGF/EGFR signaling, ERK/MAPK, p53 signaling and more.

Key Features:
· Site-specific phosphorylation profiling and screening with 269 antibodies
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Six replicates per antibody
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for analyzing two samples (untreated vs. treated)
· Featured Antibodies: 269 site-specific and phospho-specific antibodies; 6 replicates per antibody
· Reactivity: Human: 100% | Mouse: 88% | Rat: 68%
· Suitable Sample Type: Cell lysate | Tissue lysate
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
14-3-3 zeta (Ser58), 4E-BP1 (Thr36), AKT (Ser473), AKT (Thr308), AKT2 (Ser474), AMPK1 (Thr174), BAD (Ser112), BAD (Ser136), BAD (Ser155), BCL2 (Ser70), BCL2 (Thr56), BCL-XL (Ser62), BRCA1 (Ser1423), BRCA1 (Ser1524), CaMK2 (Thr286), Catenin b (Ser33), Catenin b (Ser37), Catenin b (Thr41/Ser45), Caspase 3 (Ser150), Caspase 9 (Ser144), Caspase 9 (Thr125), Caspase 9 (Tyr153),Caveolin-1 (Tyr14), CDC2 (Tyr15), CDC25A (Ser75), CDC25C (Ser216), CDK2 (Thr160), Chk1 (Ser280), Chk1 (Ser317), Chk1 (Ser345), Chk2 (Ser516), Chk2 (Thr68), c-Jun (Thr239), c-Jun (Ser243), c-Jun (Ser73), c-Kit (Tyr721), CREB (Ser133), CrkII (Tyr221), eEF2K (Ser366), EGFR (Tyr1110), eIF2A (Ser51), eIF4E (Ser209), Elk1 (Ser383), ERK3 (Ser189), ERK8 (Thr175/Tyr177), Estrogen Receptor-a (Ser167), FAK (Tyr397), FAK (Tyr861), FAK (Tyr925), FGFR1 (Tyr154), FKHR (Ser256), GSK3 alpha (Ser21), GSK3 beta (Ser9), HDAC8 (Ser39), HER2 (Tyr877), Histone H2A.X (Ser139), HSF1 (Ser303), HSP27 (Ser15), HSP27 (Ser78), HSP90B (Ser254), ICAM-1 (Tyr512), IGF-1R (Tyr1161), IkB-alpha (Ser32/36), IkB-alpha (Tyr42), IkB-beta (Ser23), IkB-epsilon (Ser22), IKK-alpha (Thr23), Integrin beta-3 (Tyr773), Integrin beta-3 (Tyr785), JAK1 (Tyr1022), JAK2 (Tyr1007), JAK2 (Tyr221), JunB (Ser259), JunB (Ser79), JunD (Ser255), Keratin 18 (Ser33), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Thr291), MEK-2 (Thr394), Met (Tyr1349), MKK3 (Ser189), MSK1 (Ser376), mTOR (Ser2448), Myc (Ser62), Myc (Ser373), Myc (Thr358), Myc (Thr58), NFkB-p100/p52 (Ser865), NFkB-p100/p52 (Ser869), NFkB-p105/p50 (Ser893), NFkB-p105/p50 (Ser907), NFkB-p105/p50 (Ser932), NFkB-p65 (Ser529), NFkB-p65 (Thr254), p21Cip1 (Thr145), p27Kip1 (Ser10), p27Kip1 (Thr187), P38 MAPK (Thr180), P38 MAPK (Tyr182), p44/42 MAPK (Thr202), p44/42 MAPK (Tyr204), p53 (Ser6), p53 (Ser315), P70S6K (Ser424), PDGF R beta (Tyr751), PDK1 (Ser241), PI3K p85a (Tyr607), PTEN (Ser380/Thr382/Thr383), Pyk2 (Tyr402), Rac1/cdc42 (Ser71), Raf1 (Ser259), Rb (Ser780), Rel (Ser503), SAPK/JNK (Thr183), Shc (Tyr349), SHP-2 (Tyr580), Smad3 (Ser425), Src (Tyr418), Src (Tyr529), STAT1 (Ser727), STAT1 (Tyr701), STAT3 (Ser727), STAT3 (Tyr705), STAT4 (Tyr693), STAT5A (Ser780), STAT5A (Tyr694), STAT6 (Thr645), STAT6 (Tyr641), Tau (Ser404), Trk B (Tyr515), TYK2 (Tyr1054), VEGFR2 (Tyr951)

Assay Outline:
The ELISA based Cancer Signaling Phospho Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.