Cell Signaling Phospho Antibody Array

The Cell Signaling Phospho Antibody Array features 304 highly specific antibodies important to 16 commonly studied cell signaling pathways. The array is designed for protein phosphorylation profiling using cell and tissue samples from human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

The Cell Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative profiling and screening of candidate biomarkers from 16 cell signaling pathways, including PI3K/AKT signaling, apoptosis, autophagy, cell cycle, ErbB, focal adhension, MAPK, p53 signaling, VEGF and more. It is designed to identify total protein changes and phosphorylation changes by comparing normal samples to treated/diseased samples. This array features site-specific and phospho-specific antibodies for studies of phosphorylation events at specific phospho sites.

Key Features:
· 304 antibodies from 16 cell signaling pathways
· Site-specific phosphorylation profiling and screening
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for analyzing two samples (untreated vs. treated)
· Featured Antibodies: 304 site-specific and phospho-specific antibodies; 6 replicates per antibody
· Reactivity: Human: 99% | Mouse: 87% | Rat: 66%
· Suitable Sample Type: Cell lysate | Tissue lysate
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
· PI3K/AKT
AKT1, AKT2, BAD, BCL2, CASP9, CCND1, CCNE1, CDK2, CKDN1A, CHUK, CREB1, EGFR1, EIF4E, EIF4EBP1, ERBB2, FOXO3, GSK3B, HSP90AB1, IGF1R, IKBKB, IKBKG, IL4R, IRS1, ITGB3, JAK1, JAK2, KDR, MAP2K1, MDM2, MET, MTOR, MYC, NFKB1, NOS3, NTRK2, PDGFRB, PDK1, PPP2CA, PRKAA1, PTEN, PTK2, RAF1, RELA, RPS6KB1, STK11, SYK, TP53, TSC2, YWHAQ
· AMPK
ACACA, AKT1, AKT1S1, AKT2, CCND1, CREB1, EEF2, EEF2K, EIF4EBP1, FOXO1, FOXO3, IFG1R, IRS1, MAP3K7, MTOR, PDPK1, PPARG, PPP2CA, PRKAA1, RPS6KB1, SREBF1, STK11, TSC2
· Apoptosis
ACTB, ACTG1, AKT1, AKT2, BAD, BAX, BCL2, BID, CASP3, CASP8, CASP9, CHUK, EIF2S1, FADD, FOS, IKBKB, IKBKG, LMNA, MAP2K1, MAP3K5, MAPK9, NFKB1, NFKB1A, PDPK1, RAF1, RELA, TP53
· Autophagy
AKT1, AKT1S1, AKT2, BAD, BCL2, BECN1, EIF2S1, IGF1R, IRS1, MAP2K1, MAP3K7, MAPK9, MTOR, PDPK1, PPP2CA, PRKAA1, PTEN, RAF1, RPS6KB1, STK11, TSC2
· Cell Cycle
ABL1, CCND1, CCNE1, CDC25C, CDK2, CDKNIA, CHEK1, CHEK2, GSK3B, HDAC1, HDAC2, MDM2, MYC, PLK1, RB1, SMAD2, SMAD3, TP53, YWHAQ
· ErbB
ABL1, AKT1, AKT2, BAD, BRAF, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CBL, CDKN1A, CRK, EGFR, EIF4EBP1, ELK1, ERBB2, GAB1, GSK3B, MAP2K1, MAP2K4, MAPK9, MTOR, MYC, PAK1, PLCG1, PTK2, RAF1, RPS6KB1, SHC1, SRC, STAT5A, STAT5B ,
· Focal Adhesion
ACTB, ACTG1, AKT1, AKT2, BAD, BCL2, BRAF, CCND1, CRK, CTNNB1, EGFR, ELK1, ERBB2, GSK3B, IGF1R, ITGB3, KDR, MAP2K1, MAKP9, MET, PAK1, PDGFRB, PDPK1, PPP1CA, PTEN, PTK2, RAF1, RASGRF1, SHC1, SRC, VASP
· Insulin
ACACA, AKT1, AKT2, BAD, BRAF, CALM1, CBL, CRK, EIF4E, EIF4EBP1, ELK1, FOXO1, GSK3B, IKBKB, IRS1, MAK2K1, MAPK9, MTOR, PDPK1, PPP1CA, PRKAA1, RAF1, RPS6KB1, SHC1, SREBF1, TSC2 ,
· JAK-STAT
AKT1, AKT2, BCL2, CCND1, CDKN1A, EGFR, IFNGR1, IL10RA, IL4R, JAK1, JAK2, MTOR, MYC, PDGFRB, PTPN11, RAF1, STAT1, STAT2, STAT3, STAT4, STAT5A, STAT5B, STAT6
· MAPK
AKT1, AKT2, BRAF, CHUK, CRK, EGFR, ELK1, ERBB2, FOS, HSPB1, IGR1R, IKBKG, KDR, MAP2K1, MAP2K4, MAP3K5, MAP3K7, MAPK14, MAPK9, MAPT, MET, MYC, NFKB1, NTRK2, PAK1, PDFGRB, RAF1, RASGRF1, RELA, RELB, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KA5, SRF, TP53, CASP3
· mTOR
AKT1, AKT1S1, AKT2, BRAF, CHUK, EIF4E, EIF4EBP1, GSK3B, IFG1R, IKBKB, IRS1, MAP2K1, MTOR, PDPK1, PRKAA1, PTEN, RAF1, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KB1, STK11, TSC2, WNT1
· NF-kappa B
BCL2, BLNK, CHUK, IKBKB, IKBKG, LCK, LYN, MAP3K7, NFKB1, NFKB1A, PLCG1, RELA, RELB, SYK, ZAP70
· p53
BAX, BCL2, BID, CASP8, CASP9, CCND1, CCNE1, CDK2, CDKNIA, CHEK1, CHEK2, MDM2, PTEN, TP53, TP73, TSC2, CASP3, CASP8
· Ras
ABL1, AKT1, AKT2, BAD, CALM1, CHUK, EGFR, ELK1, GAB1, GAB2, GRIN1, IGF1R, IKBKB, IKBKG, KDR, MAP2K1, MAPK9, MET, NFKB1, PAK1, PDFGRB, PLCG1, PTPN11, RAF1, RASGRF1, RELA, SHC1, ZAP70
· TGF-beta
MYC, PPP2CA, RPS6KB1, SMAD1, SMAD2, SMAD3, TGFBR1, TGFBR2
· VEGF
AKT1, AKT2, BAD, CASP9, HSPB1, MAP2K1, MAPK14, NOS3, PLCG1, PTK2, RAF1, SRC, VEGFR2

Assay Outline:
The ELISA based Cell Signaling Phospho Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.