Name: Calmodulin N60D - ¹³C¹⁵N labeled
Price: Inquiry

- Overview
  - MW=16.6 kDa calculated. Full length Calmodulin with the N60D mutation, cloned from human cDNA, expressed in E. coli. The protein consists of the human Calmodulin (residues 1-148, UniProtKB accession P62158) with a N60D mutation.
    
    · Available mutants
    WT – CAT # G02CLM01
    
    Shipping in Dry Ice: yes
    Labeling: 13C15N
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Human
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.
    
    Formulation
    
    1.0 mg/mL solution in Tris 20 mM pH 8.0, NaCl 150 mM, CaCl2 2 mM, Complete Protease Inhibitor Cocktail EDTA-free (Roche). The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 2980 M^-1cm^-1 calculated). It is strongly recommended to add 1 tablet of Complete Protease Inhibitor Cocktail EDTA-free (Roche) in 1000 mL of buffer in case of buffer exchange.
    
    Storage
    
    -20°C. The protein is stable at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C.
    Avoid repeated freeze/thaw cycles.
    
    Molecular Weight
    
    16.6 kDa
    
    Expression system
    
    E. coli
    
    Sequence
    
    10 20 30 40 50
    MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ
    60 70 80 90 100
    DMINEVDADG DGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY
    110 120 130 140
    ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTA
    
    * This product is intended for research use or further manufacturing only.
- Reference
  - Experimentally exploring the conformational space sampled by domain reorientation in calmodulin

Calmodulin N60D - ¹³C¹⁵N labeled

Calmodulin N60D - ¹³C¹⁵N labeled

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