Name: Carbonic Anhydrase - ¹³C¹⁵N
Price: Inquiry

- Overview
  - MW = 29.1 kDa. Full length Carbonic Anhydrase II (Carbonate dehydratase II, Carbonic anhydrase C) cloned from human cDNA and expressed in E.coli. The protein consist of the human Carbonic Anhydrase II with a mutation Ser2Ala. (Swiss prot accession: P00918). Mass spectrometry (MALDI) showed that the first aminoacid (Met) is processed during the expression.
    
    · Characteristics
    Under the above described conditions, the product can be concentrated up to 1 mM.
    
    Shipping in Dry Ice: yes
    Labeling: 13C15N
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Human
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight at about 30.0 kDa.
    
    Formulation
    
    2 mg/mL in Tris buffer 20mM pH 7.5, NaCl 150mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 54000 M^-1cm^-1).
    
    Storage
    
    -20°C. The protein is stable at 4°C for weeks and at 25°C for days. Avoid repeated freeze/thaw cycles.
    
    Molecular Weight
    
    29.1 kDa
    
    Expression system
    
    E. coli
    
    Sequence
    
    10 20 30 40 50
    MAHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS
    60 70 80 90 100
    YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL
    110 120 130 140 150
    DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG
    160 170 180 190 200
    SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP
    210 220 230 240 250
    PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL
    260
    KNRQIKASFK
    
    * This product is intended for research use or further manufacturing only.
- Reference
  - Strategies of signal assignments in paramagnetic metalloproteins. An NMR investigation of the thiocyanate adduct of the cobalt (II)-substituted human carbonic anhydrase II

Carbonic Anhydrase - ¹³C¹⁵N

Carbonic Anhydrase - ¹³C¹⁵N

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