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By-5 phosphoramidite

By-5 phosphoramidite

Catalog Number:

CAL1278194APT

Mfr. No.:

APT-1122

Price:

$315

Size:

Quantity:

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- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cyanine phosphoramidites (Cy3, Cy3.5, Cy5, Cy5.5 analogs)
    
    Alternative Name
    
    2-[5-[3,3-dimethyl-1-(5-{[(1R,4R)-4-({[bis(propan-2-yl)amino](2-cyanoethoxy)phosphanyl}oxy)cyclohexyl]carbamoyl}pentyl)-2,3-dihydro-1H-indol-2-ylidene]penta-1,3-dien-1-yl]-1,3,3-trimethyl-3H-indol-1-ium tetrafluoroborate
    
    Molecular Formula
    
    C47H67BF4N5O3P
    
    Molecular Weight
    
    867.8 g/mol
    
    Appearance
    
    Dark blue powder
    
    Other Properties
    
    1H NMR (CD3CN): Conforms to the internal standard
    31P NMR (CD3CN):
    Assay: ≥93% (31P NMR)
    Coupling test (with T10 AI157oligonucleotide): Passed
    
    Storage
    
    Keep in a dark and dry place at -20°C (Shelf Life: 1 year)
    
    * Research Use Only
- Applications
  - Application Description
    
    Dilution: dilute 100 mg of By-5 phosphoramidite in 1.2 mL of anhydrous acetonitrile to obtain 0.1M solution
    
    Oxidation: 0.02 M iodine is recommended
    
    Coupling: 3 minute coupling time is recommended
    
    Deprotection: 24 hours at room temperature in conc. ammonia is recommended

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