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By-3.5 phosphoramidite

By-3.5 phosphoramidite

Catalog Number:
CAL1278195APT
Mfr. No.:
APT-1121
Price:
$307
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      • Overview

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      • Properties
        • Categories
          Cyanine phosphoramidites (Cy3, Cy3.5, Cy5, Cy5.5 analogs)
          Alternative Name
          2-[3-[3-butyl-1,1-dimethylbenzo[e]indol-2-ylidene]prop-1-en-1-yl]-1,1-dimethyl-3-(5-{[(1r,4r)-4-{[(2-cyanoethoxy)(diisopropylamino)phosphanyl]oxy}cyclohexyl] carbamoyl}pentyl)benzo[e]indol-3-ium tetrafluoroborate
          Molecular Formula
          C56H75BF4N5O3P
          Molecular Weight
          984.0 g/mol
          Appearance
          Metallic purple powder
          Other Properties
          1H NMR (CD3CN): Conforms to the internal standard
          31P NMR (CD3CN): Conforms to the internal standard
          Assay: ≥98% (31P NMR)
          Coupling test (with T10 AI157oligonucleotide): Passed
          Storage
          Keep in a dark and dry place at -20°C (Shelf Life: 1 year)

          * Research Use Only

      • Applications
        • Application Description
          Dilution: dilute 100 mg of By-3 phosphoramidite in 1.2 mL of anhydrous acetonitrile to obtain 0.1M solution

          Oxidation: 0.02 M iodine is recommended

          Coupling: 3 minute coupling time is recommended

          Deprotection: 24 hours at room temperature in conc. ammonia is recommended

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