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By-3 phosphoramidite

By-3 phosphoramidite

Catalog Number:
CAL1278196APT
Mfr. No.:
APT-1120
Price:
$307
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      • Overview

        • Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Cyanine phosphoramidites (Cy3, Cy3.5, Cy5, Cy5.5 analogs)
          Alternative Name
          3,3-dimethyl-1-(5-{[(1R,4R)-4-({[bis(propan-2-yl)amino](2-cyanoethoxy)phosphanyl}oxy)cyclohexyl]carbamoyl}pentyl)-2-[3-[1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium tetrafluoroborate
          Molecular Formula
          C45H65BF4N5O3P
          Molecular Weight
          841.8 g/mol
          Appearance
          Metallic green or red powder
          Other Properties
          1H NMR (CD3CN): Conforms to the internal standard
          31P NMR (CD3CN): Conforms to the internal standard
          Assay: ≥94% (31P NMR)
          Coupling test (with T10 AI157oligonucleotide): Passed
          Storage
          Keep in a dark and dry place at -20°C (Shelf Life: 1 year)

          * Research Use Only

      • Applications
        • Application Description
          Dilution: dilute 100 mg of By-3 phosphoramidite in 1.2 mL of anhydrous acetonitrile to obtain 0.1M solution

          Oxidation: 0.02 M iodine is recommended

          Coupling: 3 minute coupling time is recommended

          Deprotection: 24 hours at room temperature in conc. ammonia is recommended. By 3 is compatible with UltraFast deprotection (AMA 10 minutes at 65 °C) if Ac-dC was used.

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