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Overview
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BoxA 2D is the mutant form of BoxA (DNA binding domains of HMGB1 protein) in which the two cysteines are replaced with aspartic acid.
It consists of 89 amino acids and has a calculated molecular mass of approximately 10.4 kDa.
BoxA 2D in migration assay cannot block the HMGB1 induced migration of fibroblasts.
It contains only trace amounts of LPS (<0.4 ng/mg protein).
BoxA 2D variant can be used as negative control of BoxA wt.
It has the sequence:
["MGKGDPKKPR GKMSSYAFFV QTDREEHKKK HPDASVNFSE FSKKDSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF" ]
Molecular Mass: BoxA 2D from HMGB1 consists of 89 amino acid and has a calculated molecular mass of approximately 10.4 kDa.
Purity: The purified protein is >95% homogeneous (electrophoresis). It contains no nucleic acids.
Endotoxin Level: The purified protein is free from LPS (Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL). The product contains <0.006% v/v of Triton X-114 due to LPS removal procedure. The remaining traces of Triton X-114 can be removed upon request.
Buffer & Reconstitution: the lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl.
Storage: the protein is shipped lyophilized. Once resuspended can be stored frozen at -20°C.Please contact us at for specific academic pricing.
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- Properties
- Reference
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Overview