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Overview
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Bovine Serum Albumin (BSA) also referred as Fraction V (as it has been originally found in the fifth ethanol fraction using Cohn′s cold ethanol precipitation method) is a Serum Albumin Protein derived from bovine serum with a molecular weight of 66.4kDa. It is a water-soluble protein which consists of 583 amino acids. It consists of three homologous domains which have been formed by six α-helices. Albumin is the most abundant protein of the serum (about 60% of the total protein content of plasma). which regulates the osmotic pressure and pH of blood, and transports a wide variety of endogenous and exogenous compounds such as fatty acids, metal, amino acids, steroids and drugs.BSA, Acetylated Molecular Biology Grade has been nucleases inactivated by acetylation and is suitable for blocking Southern blots. Acetylated BSA should not be usedas a protein standard in protein determinations assays like Bradford and Lowery assays, since acetylation of tyrosine residues prevents color development.
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