BlasTaq™ HotStart DNA Polymerase

BlasTaq™ HotStart DNA Polymerase

Catalog Number:
PER1279055APP
Mfr. No.:
APPL_G595
Price:
$315
  • Size:
    400 rxn (200 ul)
    Quantity:
    Add to Cart:
      • Overview
        • Eliminate non-specific amplification and primer-dimer formation!
          BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.
          During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.

          Product Features:
          No non-specific amplification or primer-dimers
          Improves yield of desired product
          Simplifies reaction setup
          Earn 2X the rewards points

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          PCR Polymerases
          Storage
          Store at -20°C.

          * For research use only. Not for diagnostic or therapeutic use.

      • Reference
        • Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans" DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.

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