Name: Biotin-SS-Sulfo-OSu
Price: 779.00 USD

- Overview
  - Biotin-SS-Sulfo-Osuis a biotin molecule containing N-hydroxysuccinimide active ester groups, whichcan be used to introduce biotin to the amino groupsof proteins such as antibodies andenzymes. Thecleavage of the linker site for unlike biotinylatedagent having only alkyl chain linkers, Biotin-AC5Sulfo-OSu or Biotin Sulfo-OSu,but disulfide group introducedinto the linker site is easily reduced by a reducing agent is possible. After labelingmolecules using Biotin-SS-Sulfo-OSu on column coatedwith avidin or streptavidin, the molecules can be recovered by cleavage of the linker site by reduction operation.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Biotin Labeling Reagent
    
    Alternative Name
    
    N-[[2-(Biotinylamino)ethyl]dithiopropionyloxy]sulfosuccinimide, sodium salt
    
    CAS Number
    
    325143-98-4
    
    Molecular Weight
    
    C19H27N4NaO9S4=606.69
    
    Appearance
    
    White to almost white powder
    
    Purity
    
    (HPLC): ≧ 80.0 %
    
    Storage
    
    -20°C, Protect from moisture
    
    * For research use only
- Reference
  - 1) M. Shimkus, J. Levy and T. Herman, "A Chemically Cleavable Biotinylated Nucleotide: Usefulness in the Recovery of Protein-DNA complexes From Avidin Affinity Columns", Proc. Natl. Acad. Sci. USA, 1985, 82, 2593.
    2) G. Nikov, V. Bhat, J. S. Wishnok and S. R. Tannenbaum, "Analysis of Nitrated Proteins by Nitrotyrosine-specific Affinity Probes and Mass Spectrometry", Anal. Biochem., 2003, 320(2), 214.
    3) S. B. Scheurer, C. Roesli, D. Neri and G. Elia, "A Comparison of Different Biotinylation Reagents, Tryptic Digestion Procedures, and Mass Spectrometric Techniques for 2-D peptide Mapping of Membrane Proteins", Proteomics, 2005, 5, 3035.
    4) J. C. Timmer, M. Enoksson, E. Wildfang, W. ZHU, Y. Igarashi, J-B. Denault, Y. Ma, B. Dummitt, Y-H. Chang, A. E. Mast, A. Eroshkin, J. W. Smith, W. A. Tao and G. S. Salvesen, "Profiling Constitutive Proteolytic events in vivo", Biochem. J., 2007, 407, 41.
    5) S. J. Lee, K-H. Kim, J. S. Park, J. W. Jung, Y. H. Kim, S. K. Kim, W-S. Kim, H-G. Goh, S-H Kim, J-S. Yoo, D-W Kim and K. P. Kim, "Comparative Analysis of Cell Surface Proteins in Chronic and Acute Leukemia Cell Lines", Biochem. Biophys. Res. Commun., 2007, 357, 620.

MSDS

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Manual

Biotin-SS-Sulfo-OSu

Biotin-SS-Sulfo-OSu

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