Biotin-PE-maleimide - Amerigo Scientific

- Overview
  - The avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of the 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric or fluorometric detection of the antigen is performed using enzyme or fluorophore-labeled avidin or streptavidin. Maleimide biotins react with thiol compounds, such as proteins or peptides with sulfhydryl groups, at pH 7-7.5. Maleimide reacts with sulfhydryl group to create a thioether bond. Although other maleimide biotin reagents must be dissolved in DMSO, DMF, or alcohol, Biotin-PE-maleimide can be solubilized in PBS at pH 7.4 to prepare 2 mM solution without using an organic solvent. The reactivity of maleimide with sulfhydryl groups is higher than that of bromoacetamide, therefore the required concentration of maleimide biotin is much lower than that of bromoacetamide biotins. Stock solutions of Biotin-PEmaleimide and Biotin-PEAC5-maleimide in DMSO are stable for one year at -20°C.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Biotin labeling
    
    Molecular Formula
    
    C20H29N5O4S
    
    Molecular Weight
    
    435.54
    
    Appearance
    
    white or slightly yellow powder
    
    Purity
    
    ≥90.0% (HPLC)
    
    Storage
    
    -20°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Reference
  - 1) Hashida, M. Imagawa, S. Inoue, K. H. Ruan and E. Ishikawa , Mor Useful Maleimide Compounds for the Conjugation of Fab'-to Horseradish Peroxidase throuth Thiol Groups in the Hinge, J. Appl. Biochem., 1984, 6, 56.
    
    2) E. Ishikawa, M. Imagawa, S. Hashida, S. Toshitake, Y. Hamaguchi and T. Ueno, Enzyme-labeling of Antibodies and their Fragments for Enzyme Immunoassay and Immunohistochemical Staining, J. Immunoassay, 1983, 4, 209.
    
    3) H. -J. Friesen, P. Hermentin and P. Gronski, Novel Maleimido-Biotins for the Selective Biotinylation of Sulfhydrils, Protides Biol. Fluids, 1987, 34, 43.
    
    4) E. Ishikawa, S. Hashida, T. Kohno, T. Kotani and S. Ohtani, Modification of Monoclonal Antibodies with Enzymes, Biotin, and Fluorochromes and Their Applications, Immunol. Ser., 1987, 33, 113.
    
    5) R. B. del Rosalio and R. L. Wahl, Disulfide Bond-targeted Radiolabeling : Tumor Specificity of a Streptavidine-biotinylated Monoclonal Antibody Complex, Cancer Res.(Suppl.), 1990, 50, 804S.

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