Biosci™ Lymphocyte Separation Tube for Human Peripheral Blood

This product is used for cell separation and purification by density gradient sedimentation according to the differences in cell densities with the help of the separation medium and centrifugation. The cell gradient density separation medium produces a certain density gradient to pour the anticoagulant blood into the separation tube. After centrifugation, erythrocytes and granulocytes sink to the bottom of the tube; PBMCs (peripheral blood mononuclear cells, including lymphocytes and monocytes) float on the surface of the separation medium, and a small part of cells may suspend in the separation medium. Lymphocytes can be separated from the peripheral blood by absorbing the cells on the surface of the separation medium.

Principle of the Procedure
The density of mononuclear cells (lymphocytes and monocytes) in peripheral blood is between 1.075-1.090g/ml, which is different from that of red blood cells, multinuclear leucocytes and platelets.The densities of red blood cells and granulocytes are relatively large(about 1.092g/ml) and the density of platelets is between 1.030~1.035g/ml.Therefore, Human Lymphocyte Separation Medium is used to generate a certain degree of density gradient, and the diluted whole blood is smoothly paved on the separation medium. After centrifugation, the red blood cells and granulocytes sink to the bottom of the tube due to their large density; Since their densities are less than or equal to the density of separation medium, lymphocytes and monocytesare found at the surface of separation medium. There may also be a small amount of cells suspended in the separation medium. By sucking the cells on the surface of separation medium, mononuclear cells can be separated from peripheral blood.

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