Biosci™ Mouse Lymphocyte Separation Medium

Mouse Lymphocyte Separation Medium is a new generation of density gradient separation medium developed by Shenzhen Dakewe Bio-engineering Co., Ltd. The main component is iodixanol, with a molecular weight of 1,550. It is a completely chemically inert, non-biotoxic iodide that does not bind with any known biological function protein, does not interfere with any cell surface membrane protein, does not inhibit enzyme activity and does not interfere with antigen-antibody responses. The lymphocyte separated by this product is with high purity, good state and high yield. The operation of Mouse Lymphocyte Separation Medium is simple, easy to learn and does not need too much experience.

Our study showed that the number and quality of mouse’s spleen lymphocytes did not change significantly after 1 hour of exposure to the separation medium, and the subsequent ELISPOT test results were completely consistent with those of the control group.

Principle of the Procedure
The density of mononuclear cells (lymphocytes and monocytes) in peripheral blood is between 1.075-1.090g/ml, which is different from that of red blood cells, multinuclear leucocytes and platelets.The densities of red blood cells and granulocytes are relatively large(about 1.092g/ml) and the density of platelets is between 1.030~1.035g/ml. Therefore, Human Lymphocyte Separation Medium is used to generate a certain degree of density gradient, and the diluted whole blood is smoothly paved on the separation medium. After centrifugation, the red blood cells and granulocytes sink to the bottom of the tube due to their large density; Since their densities are less than or equal to the density of separation medium, lymphocytes and monocytesare found at the surface of separation medium. There may also be a small amount of cells suspended in the separation medium. By sucking the cells on the surface of separation medium, mononuclear cells can be separated from peripheral blood.

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