Overview

Bestdex G-25 series resin is made by dextran as raw material and chlorinated propylene oxide as cross-linking agent. Featuring hydrophilicity and multiple pore sizes, the product is widely used for buffer exchange、desalting and small molecule removal of bio-organism samples(protein, polysaccharide and nucleic acid). It can also be used for separation of peptide, oligonucleotides as well as the purification of antibiotics, chemical synthesized medication and natural organism. Besides, application to nanotechnology and sewage disposal is becoming increasingly commonplace.Functioning as molecular sieves, Bestdes G-25 series separate molecules in various sizes according to the variety in retention time of molecules in the column. Bestdex G-25 is divided into four types based on particle size: Bestdex G-25 C (coarse), Bestdex G-25 M (Medium), Bestdex G-25 F(Fine), and Bestdex G-25 SF (Super fine).

Product		Bestdex G-25 C	Bestdex G-25 M	Bestdex G-25 F	Bestdex G-25 SF
Appearance		White to yellowish spherical powder
Matrix		Cross-linking dextran
Particle size (dry) ⁺		100~300μm	50~150μm	20~80μm	20~50μm
Particle size (wet)⁺⁺		160~500μm	80~240μm	30~130μm	30~80μm
Average particle size (wet)⁺⁺		320μm	140μm	90μm	50μm
Max. flow velocity (cm/h)⁺⁺⁺		650	430	240	110
Separation range	Linear molecule	100D~5KD
	Globular molecule	1KD~5KD (10~45 amino acid)
	nucleotide	<8bp
Swelling ratio (mL /g dry powder)		2.5
Volume of swelling swelling resin per gram of dry powder		4~6mL (water) ~4.5mL (0.5M NaCl)
pH stability		2~13
Chemical Stability		Stable in common aqueous buffers: 0.2M NaOH, 0.2M HCl, 1MHAc, 6M GuHCl,8M Urea, 1%SDS, 24% ethanol, 30% propanol, 30% acetonitrile

+ Particle size is normally distributed, and particles within this range account for more than 80% of the total.
++ The particle size in different solutions will be different, this data is the particle size in PBS
+++ BXK26/70, Column height 60cm, pressure 0.3MPa measured

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