BenzoNuclease® ELISA Kit

BenzoNuclease® is a genetically engineered endonuclease from Serratia marcescens.
BenzoNuclease® acts as an endonuclease that degrades both DNA and RNA—whether single stranded, double-stranded, linear, circular or supercoiled. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphateterminated oligonucleotides, which are three to five bases in length.
BenzoNuclease® is an efficient enzyme for removing all forms of DNA and RNA from biological products. In processed biological products, there may be traces of BenzoNuclease® residues, and these traces of residues will have a certain impact on the application of subsequent biological products. The residual amount of BenzoNuclease® in biological products is one of the important indicators to measure the quality of biological products.
This kit can detect and quantitatively analyze the BenzoNuclease® residues in recombinant viral vectors and vaccine preparations with high sensitivity and specificity.
Reaction Principle
This kit adopts the double-antibody sandwich ELISA method. The BenzoNuclease capture antibody is pre-coated. After adding the sample, the sample is captured to form an antibody-antigen complex, and then the HRP-conjugated BenzoNuclease detection antibody is added to form an antibody-antigen-antibody "sandwich" complex. Finally, TMB substrate was added for color development. After the reaction was terminated, the absorbance value (OD value) was read at a wavelength of 450 nm. The content of BenzoNuclease in the sample was positively correlated with the OD value.

Please contact us at for specific academic pricing.