Benzamidine Bestarose 4FF - Affinity Resins

Benzamidine Bestarose 4FF - Affinity Resins

Catalog Number:
CM01502180BES
Mfr. No.:
AA0291-AA111314
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      • Overview
        • Benzamidine Bestarose 4FF is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 4FF agarose resin. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (such as trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for purifying such substances.

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      • Properties
        • Categories
          Affinity Resins
          Other Properties
          Appearance: White slurry
          Matrix: Cross-linked agarose, 4%
          Particle size+: 45~165μm
          Functional group: P-aminobenzidine
          Ligand concentration: ≥12μmol Ligand/mL resin
          Dynamic binding capacity: ≥35mg Trypsin/mL packed resin
          Max. pressure: 0.3MPa
          Pressure flow velocity: >150cm/h (0.1MPa BXK50/30, H=25cm, 20°C)
          Chemical stability: Stable in common aqueous buffers: 6M GuHCl, 8M Urea, PH =1, 2, 3, 4 hydrochloric acid solution, 0.025M borax solution with pH=8, 9, 10, 11
          pH stability: 2~8 (working), 1~9 (CIP)
          Storage++: 2~8°C, 20% ethanol + 50mM NaAc (pH4.0) or 2% benzyl alcohol + 50mM NaAc (pH 4.0)
          Recommended flow velocity: 30-300cm/h

          +Particle size is normally distributed, and particles within this range account for more than 95% of the total.
          ++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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