BcMag™ Peptide conjugation kit (II)

It is frequently helpful to immobilize peptides via functional groups other than amines. The thiol group, in particular, can be employed to guide coupling processes away from active centers or binding sites on specific protein molecules. Using a unique active group for site-directed immobilization is beneficial for correct orientation, such as sulfhydryl on a single terminal cysteine in a peptide for a small antigen. BcMag™ Peptide Conjugation Kit is designed to enable fast, efficient, and covalent immobilization of small peptides through their sulfhydryl groups (-SH) for affinity purification procedures. BcMag™ Cleavable Iodoacetyl-activated Magnetic Beads are recommended conjugation of small peptides because the long-arm hydrophilic linker may reduce steric hindrance. Since the active iodoacetyl group is linked with the beads through a built-in cleavable disulfide linker, reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads, and only a small sulfhydryl group is attached to ligand after affinity purification. BcMag™ Cleavable Iodoacetyl-activated Magnetic Beads are uniforms and silica-based superparamagnetic beads coated with a high density of Iodoacetyl or cleavable Iodoacetyl functional groups on the surface. At physiological to alkaline circumstances (pH 7.2 to 9) in either aqueous or organic solvents 20- 30% DMSO or DMF, iodoacetyl-activated supports react with sulfhydryl groups, resulting in stable thioether bonds. These reactions are often carried out in the dark to prevent the formation of free iodine, which can react with tyrosine, histidine, and tryptophan residues.

Workflow
The Iodoacetyl magnetic beads work perfectly as solid support for various bioseparations to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.

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