BcMag™ One-Step Fungi &Yeast DNA Purification Kit

Fungi and yeast are essential microorganisms in many research fields, including medical, agricultural, and environmental sciences. However, their DNA extraction can be a challenging and time-consuming process. Standard procedures for extracting fungi and yeast DNA can be exceedingly Time consuming and labor intensive. Specific applications, such as fungi, and yeast genotyping, demand a lengthy DNA extraction method. For example, traditional fungi and yeast DNA extractions need a 4-hour to overnight digestion with proteinase K, followed by phenol: chloroform extraction and alcohol precipitation. Other techniques, such as silica bind and elute kits, have recently become available. Those techniques are based on positive chromatography purifying selection. High chaotropic salt concentrations, such as guanidine hydrochloride, bind DNA to silica. The silica column or beads is washed with a salt/ethanol solution after nucleic acid binding to eliminate additional biomolecules from the sample. Finally, the column or beads is eluted using Tris elution buffer or water to remove the pure DNA. Such bind-wash-elute procedures are time-consuming, requiring multiple washing and spinning steps. Repetitive spin steps can cause considerable DNA loss (20-40%) and shearing. Furthermore, chaotropic salts and other impurities can easily pass through the eluted DNA or RNA, compromising ultimate purity and quantification as well as downstream enzymatic activities like PCR. Bioclone has developed a kit called ” One-Step Fungi and Yeast DNA Purification kit ” to tackle the challenges associated with extracting DNA from fungi and yeast. The One-Step Fungi & Yeast DNA Purification Kit is an essential tool for efficient and rapid DNA extraction from microorganisms such as fungi and yeast. Its unique proprietary magnetic bead and buffer system offers gentle cell lysis and simultaneous impurity removal, avoiding harsh conditions and toxic chemicals. The one-step purification technique allows the simultaneous processing of many samples, increasing DNA integrity and reducing DNA loss. The purified genomic DNA is of the highest quality and suitable for various downstream applications such as qPCR. With this kit, researchers can streamline their DNA extraction process, saving time and reducing cost.

Please contact us at for specific academic pricing.