BcMag™ Hydrazide-Activated Terbium Fluorescent Magnetic Beads

Our Hydrazide-Activated Terbium Fluorescence Magnetic Beads offer high sensitivity and overcome limitations of conventional fluorophores. These magnetic microspheres combine preactivated hydrazide active groups, time-resolved fluorescence dyes, and magnetic characteristics, resulting in highly sensitive assays. The beads also overcome common issues like high background signals and sensitivity to environmental factors, exhibit low fluorescence intermittency, and avoid aggregation due to hydrophobicity.

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Background

BcMag™ TR-FRET (Time-Resolved FRET) Assay
BcMag™ TR-FRET Assay, in contrast to typical FRET (Förster Resonance Energy Transfer) assays, uses time-resolved Fluorescence magnetic beads (BcMag™ TR-Magnetic Beads) as the donor fluorophore. The donor and acceptor can be two proteins, two DNA strands, an antigen, an antibody, or a ligand and its receptor. After a reasonable time delay (usually 50 to 100 s), a signal is generated by fluorescence resonance energy transfer between a donor and an acceptor molecule when they are close and monitored in a time-resolved way. In BcMag™ TR-FRET Assay, a trace amount of analytes can be easily enriched from the complex by TR-Magnetic Beads, resulting in higher sensitivity. This assay practically eliminates all fluorescence backgrounds caused by the sample and plastic microplate, as well as by direct acceptor excitation. As a result, the signal-to-noise ratios of the BcMag™ TR-FRET Assay are very high, and the background is quite low. Furthermore, the assay does not need washing steps. BcMag™ TR-FRET Assay offers substantial advantages to bioassays in high throughput screening, such as assay flexibility, dependability, increased assay sensitivity, higher throughput, and fewer false positive/false negative results.
Terbium cryptate fluorophore is an efficient Fluorescence label due to its distinct specific properties. It is excited at 320nm and emits green fluorescence at 545nm, with a long fluorescence lifetime (1050 μsec) and large stokes shifts (220 nm). By taking advantage of these properties, time-resolved fluorescence measurement can dramatically reduce the fluorescence background from the sample and increase the signal-to-noise ratio to offer detectability better than one order of magnitude than conventional Fluorescence dyes. BcMag™ Hydrazide-activated Terbium Fluorescence Magnetic Beads are excellent donors used in TR-FRET assays.