BAPTA [CAS 85233-19-8(free acid)], ≥95.0% (Titration)

BAPTA [CAS 85233-19-8(free acid)], ≥95.0% (Titration)

Catalog Number:
CFA1108172DOJ
Mfr. No.:
B019-10
Price:
$378
  • Size:
    500 mg
    Quantity:
    Add to Cart:
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      • Overview

        • BAPTA is a calcium-selective chelator developed by Dr. Tsien. It has logKCa=6.97 and logKMg=1.77. The basic chelating unit resembles that of EGTA, but the two aliphatic nitrogen atoms are replaced by aromatic nitrogen. Thus, BAPTA is not protonated at physiological pH. BAPTA possesses pKa3=5.47 and pKa4=6.36. This property indicates that the deprotonation step is not included in its calcium complexation step, and it has a higher complexation rate than EGTA because it is not affected by proton interference. BAPTA-AM is an acetoxymethyl ester derivative of BAPTA that can be easily loaded into cells using the AM method. BAPTA-AM is useful for controlling the intracellular calcium concentration.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Intracellular Fluorescent Probes
          CAS Number
          85233-19-8(free acid)
          Molecular Formula
          C22H20K4N2O10
          Molecular Weight
          628.79
          Appearance
          White powder or crystalline powder
          Purity
          ≥95.0% (Titration)
          Storage
          ambient temperature
          Shipping
          ambient temperature

          * For research use only

      • Reference

        • 1) R. Y. Tsien, New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures. Biochemistry. 1980;19:2396-2404.

          2) R. Y. Tsien, A Non-disruptive Technique for Loading Calcium Buffers and Indicators into Cells. Nature. 1981;290:527-528.

          3) J. I. Korenbrot, et al., The Use of Tetracarboxylate Fluorescent Indicators in the Measurement and Control of Intracellular Free Calcium Ions. Soc Gen Physiol Ser. 1986;40:347-363.

          4) S. M. Harrison, et al., The Effect of Temperature and Ionic Strength on the Apparent Ca-affinity of EGTA and the Analogous Ca-chelators BAPTA and Dibromo-BAPTA. Biochim Biophys Acta. 1987;925:133-143.

          5) E. W. Gelfand, et al., Dissociation of Unidirectional Influx of External Ca2+ and Release from Internal Stores in Activated Human T Lymphocytes. Eur J Immunol. 1990;20:1237-1241.

          6) J. P. Kao, et al., Active Involvement of Ca2+ in Mitotic Progression of Swiss 3T3 Fibroblasts. J Cell Biol. 1990;111:183-196.

          7) M. L. Schubert, et al., Functionally Distinct Muscarinic Receptors on Gastric Somatostatin Cells. Am J Physiol. 1990;258:G982-G987.

          8) Y. Tojyo, et al., Inhibitory Effects of Loading with the Calcium-chelator 1, 2-Bis(o-aminophenoxy)ethane-N, N, N’, N’-tetraacetic acid (BAPTA) on Amylase Release and Cellular ATP Level in Rat Parotid Cells. Biochem Pharmacol. 1990;39:1775-1779.

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