Bacteria Advanced DNA Kit

Bacteria Advanced DNA Kit

Catalog Number:
NAPK388925GEN
Mfr. No.:
GBBA004; GBBA100; GBBA300
Price:
$111
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      • Overview
        • The Presto™ gDNA Bacteria Advanced Kit is designed for rapid isolation of genomic DNA from cultured Gram (+) positive and Gram (-) negative bacteria. After directly adding into pre-filled beadbeating tubes containing zirconia/ceramic beads and lysis buffer, bacterial cells are efficiently lysed using a standard vortex or bead beating instrument. Enzymatic digestion with Lysozyme and Proteinase K is not required. The cell lysate is then mixed with a binding buffer, followed by bind, wash and elute using a spin column designed specifically for bacteria DNA extraction. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 30 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

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      • Properties
        • Details
          Purify genomic DNA within 30 minutes!
          High Yield: up to 40 μg (1 x 10⁹ Escherichia coli) and 15 µg (1 x 10⁹ Bacillus subtilis)
          High Purity gDNA: A260/A280 >1.9
          DNA Size: 20-40 kb
          Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 10⁹), 200 µl of blood and biological fluids such as plasma and urine
          Convenient: includes bead beating tubes (pre-filled with zirconia/ceramic beads) for efficient cell lysis without the use of enzymes such as Lysozyme or Proteinase K
          Spin Columns: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
          Elution volume: 30-200 µl
          Storage
          dry at room temperature (15-25°C)

          * For Research Use Only

      • Applications
        • Application
          Genomic DNA Purification
          Application Description
          PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

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