-Bacstain- Bacterial Viability Detection Kit-DAPI/PI

Bacstain- Bacterial Viability Detection Kits are a series of products for fluorescent double-staining of bacteria. By combining different types of fluorescent stain, staining images can be acquired for each index (membrane impairment, respiratory activity, and esterase activity). Bacterial viability is generally assessed by colony formation in the nutrient-agar medium. However, this requires a long culturing time, and it is hard to recognize the growth of viable but nonculturable (VNC) bacteria. However, fluorescent staining does not require bacterial culture and enables viability assessment by rapid and simple protocols.

-Bacstain- Bacterial Viability Detection Kit – DAPI/PI uses bacterial membrane damage as an index. DAPI is a minor groove binder specific to the AT sequence of DNA and permeates into bacteria to stain nucleic acids regardless of membrane damage. PI is a parallel intercalator into the DNA double helix that stains nucleic acid; it passes only through damaged bacterial membranes. Thus, this kit can be used to measure the ratio of membrane-damaged bacteria to total bacteria on the analysis of the fluorescent images from each stain.

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