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-Bacstain-AO Solution

-Bacstain-AO Solution

Catalog Number:
CFA1108152DOJ
Mfr. No.:
BS05-10
Price:
$277
  • Size:
    100 assays
    Quantity:
    Add to Cart:
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      • Overview

        • Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Bacterial Staining
          CAS Number
          65-62-2-AO
          Storage
          -20°C, protect from light
          Shipping
          ambient temperature

          * For research use only

      • Applications
        • Application Description
          Staining procedure
          1. Allow AO solutiona) to stand at room temperature for 30 minutes to thaw. Solution should be protected from light.
          2. Resuspend the organisms with PBS(-) or saline and adjust the number of cells.
          3. Add 3 μl of AO solution into the 1 mL of microbial cell suspension and vortex gently to mix. Formaldehyde-fixation can be carried out if necessary.
          4. Incubate the microbial cells at room temperature for 5 minutes.
          5. Analyze the stained-cells with a flow cytometer or a microscope. The maximum wavelengths of the dye with ssDNA are 420-460 nm for excitation and 630-650 nm for emission. The maximum wavelengths of the dye with dsDNA are 500 nm for excitation and 520 nm for emission.
          a) Since AO may be carcinogenic, be careful when handling and disposing.
      • Reference

        • 1) J. E. Hobbie, et al., Use of Nucleopore Filters for Counting Bacteria by Fluorescence Microscopy. Appl Environ Microbiol. 1997;33:1225-1228.

          2) S. F. Nishino, et al., Direct Acridine Orange Counting of Bacteria Preserved with Acidified Lugol Iodine. Appl Environ Microbiol. 1986;52:602-604.

    MSDS

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    Manual

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