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ARSA Recombinant Protein

ARSA Recombinant Protein

Catalog Number:
P001417058ABE
Mfr. No.:
Abe32-2160
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  • Size:
    20 µg
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      • Overview

        • ARSA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 512 amino acids (21-509) and having a molecular mass of 54.3kDa. ARSA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Arylsulfatase A (ARSA) hydrolyzes cerebrosidesulfate to cerebroside and sulfate. ARSA is inhibited by phosphate. The phosphate develops a covalent bond with the active site 3-oxoalanine. ARSA gene defects cause metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in various neurological symptoms and ultimately death.

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      • Properties
        • Protein Name
          Arylsulfatase A, ASA, EC 3.1.6.8, Cerebroside-sulfatase, ARSA, MLD.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 90% as determined by SDS-PAGE.
          Formulation
          The ARSA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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