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Overview
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Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells.
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Background
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence once has been transfected into cells, which is a direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells by various fluorescence-based assays. You can observe the fluorescence at 509 nm.
Our product had already been modified with ARCA with a high efficient co-transcriptional capping method, which means most of mRNA has a Cap 0 structure. It is a more mature mRNA than mRNA with no capping and maintain a more stable state which lead to higher expressing efficiency in transfected mammalian cells. ARCA obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP.
A upgraded version of ARCA EGFP mRNA is available, please check out R1007 ARCA EGFP MRNA (5-moUTP) for more details.
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- Properties
- Reference
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Overview