AQ97 High Fidelity Hot Start DNA Polymerase

AQ97 Hot Start High Fidelity DNA Polymerase is a thermostable, chimeric DNA polymerase created specifically for low-bias, high fidelity amplification of a vast range of amplicons.

AQ97 Hot Start High Fidelity DNA Polymerase delivers high-speed elongation and processivity, due to its fusion with a DNA-binding domain.

The antibody-mediated inactivation of the polymerase and exonuclease functions increases specificity of the enzyme, as well as enabling room temperature setup.

AQ97 Hot Start High Fidelity DNA Polymerase is recommended for applications, which require extremely high fidelity such as cloning/sub-cloning, NGS applications, SNP analysis and mutagenesis.

AQ97 Hot Start High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity, enabling accurate correction of base-pair mismatches. The attached DNA-binding domain enhances processivity, while the antibody-mediated inactivation ensures specificity even at room temperature setup. Together, these features yield an enzyme that consistently delivers high-fidelity, specific, and fast results with long-range capacity and low-bias amplification.

The AQ97 Hot Start High Fidelity DNA Polymerase kit includes the 5x AQ97 Buffer allowing robust amplification of long targets with low to high GC content. The kit also contains an extra tube of MgCl2 in 25 mM concentration if adjustments are required.

Addition of 1-2 M Betaine Enhancer Solutions is recommended when amplifying difficult amplicons, such as GC-rich targets, long targets, or those with complex secondary structures.

For more convenient handling AQ97 Hot Start High Fidelity DNA Polymerase is also available as a 2x master mix.

FIDELITY
The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerase error rate. Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.

Features
• 2 minute hot start
• Reaction setup at room temperature
• High fidelity: >60x Taq Fidelity
• High elongation rate: 10 sec/kb
• 3’→5’ proofreading exonuclease activity
• Long range capacity: 18 kb for gDNA and 25 kb for λ DNA
• Good coverage on DNA templates with low to high GC content

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