Apolipoprotein B (APO-B) Assay

Assay Principle
This method is based on the reaction of a sample containing human Apo B and a specific antiserum to form an insoluble complex which can be measured turbidimetrically at 340 nm. By constructing a standard curve from the absorbance of standards the concentration of apo B in the samples can be determined.

Features
Apo B Assay has a linear range of 3.6 – 240 mg/dL with good correlation with existing FDA approved product with r2 values >0.99. The Apo B Assay is not interfered by the following substances at the indicated concentrations: triglycerides at 1000 mg/dL, ascorbic acid at 10 μM, bilirubin at 40 mg/dL, bilirubin conjugate at 40 mg/dL, and hemoglobin at 1000 mg/dL.

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