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Overview
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MaxLight™ Rapid Labeling Kits Titered for ease of use Ready to label 3x100ug conjugations/kit
100ug of antibody at 0.9-1.0mg/ml
Complete and purified conjugation in 1hour with 15 hands on time >90% yield
Steps
· Everything included spin, transfer, incubate, spin, spin, done.
· No buffers, no organic solvents, no calculations Simple protocol using just a microcentrifuge and a Nanodrop or UV spec
· Incorporates the optimal number of dye per antibody
· Pure product is produced, all excess label is removed. Exclusive to MaxLight™ kits
· Multiple antibodies can be modified simultaneously with different labels
Simple Protocol
1. Prepare Antibody (~100ug, ~1mg/ml)
2. Prepare Desalting Column (Blue Cap), add antibody, centrifuge (2min)
3. Add Antibody to Labeling Column (Clear Cap), vortex, incubate (1hr, dark, RT)
4. Prepare 2 Purification Columns (Red Cap), add labeled antibody, centrifuge (2min)
5. Repeat (add antibody to Purification Column #2 (Red Cap), centrifuge (2min)
6. Flow-through is final labeled antibody, free of unconjugated dye.Please contact us at for specific academic pricing.
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- Properties
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Overview