Antibody or Protein Exatecan Conjugation Kit with VC-PAB Linker

We have designed this kit to label any antibody or protein (≥20 kDa) with Exatecan using a valine-citruline p-aminobenzylcarbamate (VC-PAB) linker. Exatecan is a synthetic analog of Camptothecin (CPT) and exhibits antineoplastic activity by inhibiting topoisomerase 1.

Using the kit components along with a user-supplied protein or antibody, the user first converts the antibody or protein into a thiol-protein through surface amine labeling. The thiol-protein is then reacted with maleimide-activated Exatecan to generate antibody- or protein-Exatecan conjugates. The final product is then purified to remove any unreacted drug.

Kit is configured to conjugate 6.67-20 nano-mole (nmol) of one (CM11436x1) or three antibodies or proteins (CM11436x3).

If you have a smaller amount of protein, a protein with a MW below 20KDa, or other customization needs, please select the customization radio button and/or fill out the information above.

Drug Information
Name: Mc-VC-PAB-Exatecan
CAS number: 2504068-28-2
Chemical formula: C53H60FN9O12
MW: 1034.10
Mechanism of action: A potent topoisomerase I inhibitor and anticancer agent. It binds to topoisomerase I (Topo I)-DNA complexes via hydrogen bonding, preventing DNA relegation and inducing DNA damage and apoptosis.
Activities: Anti-Cancer

Requirement for Antibody or Protein
Preferably >90% pure by gel electrophoresis
Total amount: 6.67-20 nano-mole (nmol) protein content as measured by UV.
Note: the accuracy of your antibody or protein measurement is the single most important factor in obtaining an optimized DAR or DPR of 2-4.

Key Features of this PerKit™
• Simple and efficient labeling of antibody or protein with Exatecan, minimizing toxin exposure.
• Features Cathepsin B-cleavable VC-PAB linker (Ref. Doronina et al., 2008, Bioconjugate Chem., 19, 1960-1963).
• Fast and easy preparation: 6 h preparation and less than 2 hours of hands-on time.
• All reagents and supplies included for preparation and purification
• Options to choose tailored services after conjugation

Please contact us at for specific academic pricing.