Antibody or Protein PBD Dimer Conjugation Kit with Val-Ala Linker

We have designed this kit to label any antibody or protein (≥20 kDa) with PBD dimer using a dipeptide (Val-Ala) linker. Pyrrolo[2,1-c][1,4]benzodiazepines (PBDs) are sequence-selective DNA minor-groove binding agents that inhibit nucleic acid synthesis. PBD dimers, with two PBD units linked via their C8 positions, are more potent DNA alkylating agents than monomers and have been used by several companies to develop antibody-drug conjugates.

Using the kit components along with a user-supplied protein or antibody, users first convert their protein into a thiol-protein through surface amine labeling. The thiol-protein is then reacted with maleimide-activated PBD dimer to generate antibody- or protein-PBD dimer conjugates. The product is then purified to remove any unreacted drug.

Kit is configured to conjugate 6.67-20 nano-mole (nmol) of one (CM11439x1) or three antibodies or proteins (CM11439x3) with the talirine (SGD-1910) PBD dimer using a valine-alanine linker.

If you have a smaller amount of protein, a protein with a MW below 20KDa, or other customization needs, please select the customization radio button and/or fill out the information above.

Please contact us at for specific academic pricing.