Anti-TNF-alpha antibody [EBS-O-174]

Anti-TNF-alpha antibody [EBS-O-174]

Catalog Number:
A001477872STJ
Mfr. No.:
STJ16102602
Price:
$451
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      • Overview
        • Please contact us at for specific academic pricing.

      • Properties
        • Host
          mouse
          Antibody Type
          monoclonal
          Isotype
          IgG1 Kappa
          Reactivity
          human; rat
          Clone
          EBS-O-174
          Immunogen
          A BALB/c mouse was immunized with human rTNF-alpha. Fusion partner: NS-1.
          Purification
          The antibody is purified from tissue culture and contains no host Ig. Affinity purified from tissue culture.
          Conjugate
          Unconjugated
          Formulation
          PBS with 0.02% Sodium Azide.
          Storage
          Store for up to 1 year at 2-8°C upon receipt.
          Concentration
          Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
          Antigen
          (UniProt:P01375)

          * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

      • Applications
        • Application
          ELISA; FC; WB; IF; IHC
        • Application Dilutions
          ELISA solid phase: 0.1-100 µg/ml, tracer: 0.001-100 µg/ml for 30 min at RT
          Flow cytometry 0.5-1.0 ug/million cells in 0.1ml
          Immunoblotting 1-2 µg/ml
          Immunofluorescence 1-2 μg/ml
          Inhibits tumor growth order Ab without azide
          Immunohistology 1-2 µg/ml for 30-60 minutes at RT, for staining of formalin-fixed tissues no suitable antigen retrieval method is known to date
          Neutralizes rTNF-alpha mediated cytotoxicity order Ab without azide
          Protects mice against toxicity of rhTNF-alpha order Ab without azide

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