Name: Anti-SSA1/Ro52 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    E3 Ubiquitin-Protein Ligase Trim21; 52 Kda Ro Protein; 52 Kda Ribonucleoprotein Autoantigen Ro/Ss-A; Ring Finger Protein 81; Ring-Type E3 Ubiquitin Transferase Trim21; Ro(Ss-A; Sjoegren Syndrome Type A Antigen; Ss-A; Tripartite Motif-Containing Protein 21 (Gene ID:6737; UniProt:P19474)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-In transfected HEK293 transiently expressing full-length Human TRIM21, a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1µg/ml. Primary incubations were for 1 hour.
    IF-Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

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