Name: Anti-Sprouty antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Protein Sprouty Homolog 1; Spry-1 (Gene ID:10252; UniProt:O43609)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Approx 37kDa band observed in Human Kidney lysates. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells. Recommended concentration: 1-3µg/ml.
    IHC-This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.