Name: Anti-SOD1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Superoxide Dismutase Cu-Zn; Superoxide Dismutase 1; Hsod1 (Gene ID:6647; UniProt:P00441)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; EIA
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20kDa observed in lysates of cell lines HEK293, HepG2 and MCF7. Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in Western blot on Mouse: Frazziano G et al. PMID: 24213612.
    IF-Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SOD1 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml