STJ70665 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-SEMA3E antibody

Catalog Number:

A001042470STJ

Mfr. No.:

STJ70665

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Semaphorin-3e (Gene ID:9723; UniProt:O15041)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:64000.
    WB-Preliminary experiments gave bands at approx 35kDa and 20kDa in Human Prostate lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 89.2kDa according to NP_036563.1. Both detected bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
    IHC-In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration, 2-4µg/ml.